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Priyadarshani AGW1, Mewan KM1, Wikramasinghe WTSS2,
and Perera UIP2*
1Department of Biotechnology, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), Sri Lanka
2Fruit Research and Development Institute, Kananwila, Horana, Sri Lanka
Abstract
Papaya (Carica papaya L.) is a commercially important tropical fruit crop exhibiting three sex types: male, female
and hermaphrodite. Because sex expression cannot be identified morphologically at the seedling stage, excess
plants are often maintained until flowering, resulting in inefficient use of land, labour and inputs. This study
evaluated the transferability and diagnostic performance of widely used sequence-characterized amplified region
(SCAR) markers for early sex identification in a Sri Lankan papaya population. Fifty randomly selected 40–45day-old
seedlings from a controlled hermaphrodite × hermaphrodite (H×H) population were screened using
SCAR-W11 and SCAR-T12 markers, with SCAR-T1 used as a positive amplification control. SCAR-W11 and
SCAR-T12 amplified a specific ~800 bp fragment in hermaphrodite plants, whereas no amplification was
observed in female plants. Morphological assessment at flowering showed complete agreement between molecular
marker-based identification and phenotypic sex classification within the evaluated dataset showing complete
agreement between molecular and morphological sex identification. These findings indicate that SCAR-W11 and
SCAR-T12 are reliable markers for distinguishing hermaphrodite and female papaya seedlings under Sri Lankan
conditions and can facilitate more efficient nursery management and breeding programs.
Keywords: Carica papaya, Hermaphrodite, Molecular markers, SCAR, Seedling screening, Sex identification
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